Fig. 6

Human breast cancer or CRC surgical resected samples were injected into the PVS of 2 dpf Tg(fli1:eGFP) zebrafish larvae. zPDXs were treated in vivo with bevacizumab and compared with untreated controls. At 4 dpi, zebrafish xenografts were imaged by confocal microscopy. The percentage of apoptosis (a, zPDX#3 *P = 0.0159, g = 1.75 and zPDX#5 *P = 0.0101, g = 1.51) and tumor size (b, *P = 0.0381, g = 1.08) were quantified. In parallel, zebrafish xenografts were analyzed in a fluorescent stereoscope to detect micrometastasis in the CHT region, followed by confocal confirmation (c). The outcomes are expressed as AVG ± SEM. The number of xenografts analyzed are indicated below the graphs. Results are from 1 independent experiment. Statistical analysis was performed using an unpaired t-test for apoptosis and tumor size and a Fisher’s exact test for micrometastasis. Statistical results: (ns) > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Cohen’s D 1988 scale of effect size with Hedges’ g correction (g): g = 0,2 low; g = 0.5 moderate; g = 0.8 high.