FIGURE

Fig. 4

ID
ZDB-FIG-200614-6
Publication
Castro et al., 2020 - Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

The hcfc1aco60/+ allele is associated with increased cell proliferation and minimal cell death. aa′ Apoptosis of Sox2+ neural precursor cells was assessed using anti-caspase 3 and anti-Sox2 antibodies at 5 days post fertilization (DPF). Representative images are depicted from sibling wildtype (Sibling) or the hcfc1aco60/+ allele (Co60/+). Arrows indicate Sox2+ Caspase+ neural precursors. b The total number of Sox2+ Caspase+ cells was quantified in wildtype siblings and the Co60/+ allele. Independently, the total number of Sox2+ was also quantified and the graph depicts the total number of Caspase positive NPCs (red bars; *p = 1.47822E−16) present within the entire Sox2+ population (gray bars; **p < 0.05). Due to the small number of total Sox2+ Caspase+ cells present, the data is depicted as total numbers of cells in the entire brain. N = 3/group. cd, c′–d′ Representative images of larvae (Sibling or Co60/+) pulsed with ethynyl-deoxyuridine (EdU) to monitor cell proliferation at 2DPF. c, d Are 20× magnifications and c′, d′ represent region in the inset at 63× magnification. e Quantification of the total number of EdU positive cells in forebrain (FB), midbrain (MB), and hindbrain (HB) of siblings and hcfc1aco60/+ larvae at 2 DPF. *p = 0.060553, **p = 0.00108, ***p = 0.006642, N = 9 per group. All error bars represent standard error of the mean

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.