FIGURE

Fig. 4

ID

ZDB-FIG-200513-15

Publication

Liu et al., 2020 - Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors

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Fig. 4

Mislocalized EYS in pomgnt1 mutant zebrafish retina was co-localized with synaptogamin-1. Retinal sections from 2-mpf zebrafish were double stained with antibodies against EYS (red fluorescence) and synaptotagmin-1 (green fluorescence). (A) Wild-type inner/outer segment layers showing connecting cilia (CC). Maximal projection and its orthogonal views of three double stained puncta are shown. EYS-immunoreactivity was co-localized with synaptagmin-1 reactivity. (B) Wild-type outer nuclear layer (ONL). Maximal projection and its orthogonal views of two double stained puncta are shown. EYS-immunoreactivity was co-localized with synaptagmin-1 reactivity. (C) Homozygous pomgnt1sny7 mutant outer nuclear layer. Maximal projection and its orthogonal views of three double stained puncta are shown. Mislocalized EYS immunoreactive puncta was co-localized with synaptotagmin-1 reactivity. Scale bar in C: 2 µm.

Expression Data

Antibody:	Ab2-syt1
Fish:	pomgnt1^{zf3359/zf3359} pomgnt1^zf3359/+
Anatomical Term:	retinal outer nuclear layer secretory vesicle
Stage Range:	Days 45-89 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pomgnt1^{zf3359/zf3359}
Observed In:	retinal outer nuclear layer secretory vesicle
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

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