FIGURE

Figure 2-figure supplement 1

ID

ZDB-FIG-200511-25

Publication

Volpatti et al., 2020 - Identification of drug modifiers for RYR1 related myopathy using a multi-species discovery pipeline

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Figure 2-figure supplement 1

Phenotypes of <italic>unc-68</italic> mutants considered for chemical screening.

(A) The ‘thrashing assay’ is performed by counting the waveforms propagated by individual C. elegans in one minute (Maryon et al., 1996). Consistent with previous reports (Maryon et al., 1996; Maryon et al., 1998), we found that unc-68 mutants thrash at a significantly lower rate than N2 (wild type) in two independent assays (n = 10 per genotype; Student’s T-test: ***p<0.001). (B) In both liquid and solid media, the DHPR inhibitor nemadipine-A (Kwok et al., 2006) induces concentration-dependent developmental growth arrest in unc-68(r1161) (strain TR2170) and unc-68(r1162) (strain TR2171) mutants. L1 larvae were plated in chemical at Day 0 (n = 20/well liquid 96-well plate; n = 50/well solid 24-well plate) and developmental stage was measured at Day 6 (liquid) and Day 5 (solid). Abbreviations: og = overgrown with larvae/eggs and food source depleted; tnc = larvae and eggs were too numerous to count, but food source not depleted; ne: no eggs have been laid (this phenotype is consistent with inhibition of egg-laying defective protein EGL-19, a DHPR, by nemadipine-A).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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