FIGURE

Fig. 1

ID

ZDB-FIG-200406-146

Publication

Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis

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Fig. 1

Retinal developmental defects in copper stressed embryos. A Measurement of eye diameter of the embryos from control, Cu²⁺- and CuNPs- stressed groups at 48 hpf, 72 hpf, and 96 hpf, respectively. B H&E staining analysis of retina of embryos from control (B1), Cu²⁺-stressed (B2) and CuNPs-stressed groups (B3) at 96 hpf. Control embryos at 96 hpf developed normal retina with a differentiated GCL (ganglion cell layer), an INL (inner nuclear layer), and an ONL (outer nuclear layer) (indicated by the red arrows). (B4) Average number of GCL cells per section/per embryo in each group (n > 3, 3–5 sections from each embryo were used for counting the GCL cells). C Expression of retinal marker genes gnat2, grk1b, grk7a, and opnlmw1 in embryos from control, Cu²⁺-stressed, and CuNPs-stressed groups. D WISH data of gnat2 in embryos from control, Cu²⁺-stressed, and CuNPs-stressed groups, respectively (D1-D3), and the percentage of embryos exhibiting reduced expression in different groups (D4). E Expression of retinal marker genes opn1sw1, opn1sw2, opn1lw1, rhodopsin, brn3b, and vsx1 in embryos from control, Cu²⁺ − stressed, and CuNPs-stressed groups. F WISH data of opn1sw2 in embryos from control, Cu²⁺-stressed, and CuNPs-stressed groups, respectively (F1-F6), and the percentage of embryos exhibiting reduced expression in different groups (F7). B1-B3, sagittal slides in eyes domain; D1-D3, dorsal view, anterior to the left; F1-F3, dorsal view, anterior to the up; F4-F6 lateral view, anterior to the left. Scale bar: A, B1-B3, and F1-F6, 100 μm; D1-D3, 200 μm **, P < 0.01; *, P < 0.05

Expression Data

Genes:	gnat2 grk1b grk7a opn1lw1 opn1mw1 opn1sw1 opn1sw2 pou4f2 rho vsx1 (all 10)
Fish:	AB
Conditions:	chemical treatment: copper(2+) chemical treatment: copper atom
Anatomical Terms:	retina whole organism
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
gnat2	AB	control	Protruding-mouth	retina	ISH
gnat2	AB	control	Day 4	whole organism	RTPCR
gnat2	AB	chemical treatment: copper(2+)	Protruding-mouth	retina	ISH
gnat2	AB	chemical treatment: copper(2+)	Day 4	whole organism	RTPCR
gnat2	AB	chemical treatment: copper atom	Protruding-mouth	retina	ISH
gnat2	AB	chemical treatment: copper atom	Day 4	whole organism	RTPCR
grk1b	AB	control	Day 4	whole organism	RTPCR
grk1b	AB	chemical treatment: copper(2+)	Day 4	whole organism	RTPCR
grk1b	AB	chemical treatment: copper atom	Day 4	whole organism	RTPCR
grk7a	AB	control	Day 4	whole organism	RTPCR

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Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment: copper(2+) chemical treatment: copper atom
Observed In:	eye retina retinal ganglion cell layer cell whole organism
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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