FIGURE

Figure 6

ID

ZDB-FIG-200325-77

Publication

Mawed et al., 2020 - Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 6

Abnormal apoptosis and inflammation response in beclin1^+/− liver. Expressions of mRNA in 16-month-old zebrafish evaluated by qRT-PCR for inflammation-related genes (A) and apoptotic-related genes (B). (C) Representative immunoblots showed the relative protein expression of tP53, IL-6. β-actin was used as the vehicle control. (D) The ratio of tP53/β-actin and IL-6/β-actin evaluated by NIH software Image J. (E) Immunofluorescence of liver tissues probed against mutated tP53. The nuclei of the necrosis hepatocytes indicated the genomic instability and DNA damage in tumor cells (yellow arrows). CV: central vein; NC: Necrosis. Data expressed as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Expression Data

Genes:	baxa caspa il1b jak3 nfkb2 rela siva1 stat3 tnfa tnfb tp53
Antibodies:	Ab2-il6 Ab13-tp53
Fish:	AB atg7^hza54/+ (AB) atg7^hza55/+ (AB) becn1^hza56/+ (AB)
Anatomical Term:	male organism liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atg7^hza54/+ (AB) atg7^hza55/+ (AB) becn1^hza56/+ (AB)
Observed In:	male organism liver
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.