FIGURE

Figure 2—figure supplement 6.

ID

ZDB-FIG-200304-9

Publication

Shahar et al., 2020 - Large-scale cell-type-specific imaging of protein synthesis in a vertebrate brain

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Figure 2—figure supplement 6.

Three-dimensional segmentation of CFP positive cells.

Click chemistry was performed to label nascent proteins. Antibody staining for CFP was performed to detect cells expressing CFP (and MetRS^L270G) across the larvae. Individual neurons were segmented in 3D according to the CFP signal, before measuring nascent proteins (in the segmented ROIs). (A) Projection of the surface of segmented neurons across a larva following semi-automatic segmentation using Zen and Imaris 9.2 software (see Materials and methods for details). (B-G) Zoom-in on a representative ROI (white frame in A showing the raw data and segmentation. Projections of ~20 microns in depth, of the same ROI from different viewpoints (demonstrated by rotating the image (B-D)) are used to show the overlay with the surface after segmentation (E-G respectively). A ROI with a relatively low density of neurons was chosen for visualization purposes. 1805 cells were segmented in this larva (A). Yellow – surfaces (segmentation), Scale bars: A = 50 μm, B-G = 5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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