Figure 3

Loss of function of Hippo TFs/TcoFs leads to a reduction of the length of motile cilia of the KV.

(A–C) Visualization of KV cilia at the 10-somite stage using an anti-acetylated tubulin antibody in (A) control embryos of the WT Tg(dusp6:GFP) line showing both KV cells (green) and cilia (red) or (B) only cilia. (C) cilia in Vgll4b morphant. Scale bar: 40 µm. (D) Length of KV cilia in Control (Ctrl) embryos and in Vgll4l, Vgll4b, Yap/Taz, Tead1a and Tead3a morphant embryos. Control (Ctrl) embryos were injected with 8 ng of Standard MO. Graph indicates the mean of cilia length, error bars the standard deviation. Statistical significance between controls and different loss-of-function conditions: two-tailed unpaired t-test. ****p≤0.0001. Numerical data and details of statistical analysis are provided in Figure 3—source data 1.

10.7554/eLife.45241.014

Numerical data for <xref rid='fig3' ref-type='fig'>Figure 3D</xref>.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: 10-13 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 10-13 somites

Phenotype Detail
Acknowledgments
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