Fig. 2

Histology of NMDA-induced thicker NFL (time gradient)

A-D: The zebrafish were treated by I.M. in NMDA and divided into 4 distinct groups with different treatment times. A: H&E staining shows representative paraffin sections (4 µm) from a wild-type control retina immersed in distilled water along with retinas treated with 100 µmol/L NMDA for 1, 3, and 7d. The dark arrow points out the thickness of each layer. B: The thickness of the NFL+GCL (y-axis) plotted against the NMDA treatment time; C: The number of GCL (y-axis) plotted against the NMDA treatment time. Error bars represent standard error of the mean (±SEM); n=6. (unpaired t-test, ^aP<0.05, ^bP<0.01, and ^cP<0.001 compared with control); D: TUNEL staining of NMDA treatment time points and control retinas. White arrows point to apoptotic cells. NFL: Nerve fiber layer; GCL: Ganglion cell layer; IPL: Inner plexiform layer; INL: Inner nuclear layer; OPL: Outer plexiform layer; ONL: Outer nuclear layer; PRL: Photoreceptor layer. Magnification is 40×. Scale bar, 50 µm.