FIGURE

Figure 3

ID

ZDB-FIG-190723-255

Publication

Iwasaki et al., 2019 - Essential role of prostaglandin E₂ and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis

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Figure 3

Expression of genes involved in lymphatic specification. (A–H) Relative expression levels (at 24 and 36 hpf) of genes involved in lymphatic specification were quantified by RT-qPCR in morphants injected with Cont MO or EP3 MO1. Values are shown relative to the value obtained with Cont MO at 24 hpf. Each value represents the mean ± SEM (N = 3). *P < 0.05, **P < 0.01 vs Cont MO at each corresponding time. (I,J) Expression of nr2f2 was analyzed by WISH in morphants at 24 and 36 hpf. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Expression Data

Genes:	apln bmp2b ccbe1 flt4 nr2f2 sox18 vegfc wnt5b
Fish:	WT WT + MO1-ptger3
Knockdown Reagent:	MO1-ptger3
Anatomical Terms:	posterior cardinal vein spinal cord whole organism
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ptger3
Knockdown Reagent:	MO1-ptger3
Observed In:	posterior cardinal vein whole organism
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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