FIGURE

Fig. 3

ID
ZDB-FIG-190723-1310
Publication
Liu et al., 2019 - Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish
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Fig. 3

Loss of cilia genes induces hematopoietic stem and progenitor cell (HSPC) defects. a Whole-mount in situ hybridization (WISH) results of HSPC markers (runx1 and cmyb) in the aorta-gonad-mesonephros (AGM) region in control and fsd1 morphants at 36 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb in DA. b Western blotting showing the protein level of Runx1 in control and fsd1 morphants at 26 hpf. c WISH analysis showing the expression of HSPC markers runx1 and cmyb (red arrowheads) in the DA in fsd1−/−. d Western blotting showing the protein level of Runx1 in fsd1−/−. e Representative images showing runx1 and cmyb expression in control, pkd2, kif3a, and ift88 morphants at 33 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb. f WISH analysis showing the expression of HSPC marker runx1 in the DA in pkd2−/− or ift88−/− with a sub-effective dose of pkd2 MO (0.5 ng per embryo, pkd2 MOlow) or ift88 MO (0.8 ng per embryo, ift88 MOlow). The red arrowheads indicate the expression of HSPC marker runx1. Scale bars, 100 µm
Expression Data
Genes:
Fish: (all 8)
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Expression Detail
Antibody Labeling
Phenotype Data
Fish: (all 7)
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Phenotype Detail
Acknowledgments
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