PUBLICATION

Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish

Authors
Liu, Z., Tu, H., Kang, Y., Xue, Y., Ma, D., Zhao, C., Li, H., Wang, L., Liu, F.
ID
ZDB-PUB-190425-9
Date
2019
Source
Nature communications   10: 1839 (Journal)
Registered Authors
Liu, Feng, Liu, Zhibin, Ma, Dongyuan, Wang, Lu, Zhao, Chengtian
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cilia/genetics
  • Cilia/metabolism*
  • Embryo, Nonmammalian
  • Embryonic Development/physiology
  • Hemangioblasts/cytology
  • Hemangioblasts/metabolism
  • Hematopoiesis/physiology
  • Hematopoietic Stem Cells/physiology*
  • Models, Animal
  • Receptors, Notch/metabolism*
  • Signal Transduction/physiology*
  • Zebrafish/physiology
  • Zebrafish Proteins/metabolism*
PubMed
31015398 Full text @ Nat. Commun.
Abstract
Hematopoietic stem and progenitor cells (HSPCs) are capable of producing all mature blood lineages, as well as maintaining the self-renewal ability throughout life. The hairy-like organelle, cilium, is present in most types of vertebrate cells, and plays important roles in various biological processes. However, it is unclear whether and how cilia regulate HSPC development in vertebrates. Here, we show that cilia-specific genes, involved in primary cilia formation and function, are required for HSPC development, especially in hemogenic endothelium (HE) specification in zebrafish embryos. Blocking primary cilia formation or function by genetic or chemical manipulations impairs HSPC development. Mechanistically, we uncover that primary cilia in endothelial cells transduce Notch signal to the earliest HE for proper HSPC specification during embryogenesis. Altogether, our findings reveal a pivotal role of endothelial primary cilia in HSPC development, and may shed lights into in vitro directed differentiation of HSPCs.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping