ZFIN ID: ZDB-FIG-190716-21
Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation. American journal of physiology. Regulatory, integrative and comparative physiology   316(4):R395-R405 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 5

Peripheral motor axons appear defasciculated in hyperglycemic larvae. A and B: following the schedule of treatment described in Fig. 1A, axons (red, acetylated α-tubulin) appear defasciculated in the ins:NTR metronidazole (MTZ)-treated group (B, arrow) compared with the ins:NTR control group (A, asterisk). Dashed lines outline the spinal cord. NTR, nitroreductase. Scale bar = 50 μm. C: schematic of the areas in which axonal outgrowths were quantified: left dorsal muscle (LDM), right dorsal muscle (RDM), left ventral muscle (LVM), right ventral muscle (RVM), and spinal cord (sc). D: no differences were observed between segments within the groups. Number of axonal outgrowths was significantly greater in MTZ-treated (gray bars) than control (white bars) segments. MN, motor nerve. ****P < 0.0001.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC jh4Tg control Days 7-13 motor neuron axon
IHC jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 motor neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 motor neuron axon defasciculated, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.