ZFIN ID: ZDB-FIG-190716-20
Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation. American journal of physiology. Regulatory, integrative and comparative physiology   316(4):R395-R405 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 6

Acute hyperglycemia affects myelination and Schwann cell count along the motor nerve. A and B: lateral view of the spinal cord of control and metronidazole (MTZ)-treated ins:NTR;Tg(mbp:EGFP-CAAX) fish following the schedule of treatment described in Fig. 1A. NTR, nitroreductase; mbp, myelin basic protein; EGFP, enhanced green fluorescent protein. C: length of myelinated nerves was significantly shorter in MTZ-treated than control ins:NTR;Tg(mbp:EGFP-CAAX) fish. ****P < 0.0001. D and E: cross sections through ins:NTR zebrafish labeled with an antibody against Sox10 (red) and acetylated α-tubulin (green) in control and MTZ-treated fish. F: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads a modest decrease in Sox10+ cells (D′′ and E′′, arrows) along the motor axons (D′ and E′) in MTZ-treated compared with control fish. *P < 0.05. Scale bars = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh4Tg; ue2Tg control Days 7-13 myelin accumulating cell IFL
jh4Tg; ue2Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 myelin accumulating cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC jh4Tg control Days 7-13 motor neuron axon
IHC jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 motor neuron axon
Ab2-sox10 IHC jh4Tg control Days 7-13 myelinating Schwann cell
IHC jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 myelinating Schwann cell
Phenotype Details
Fish Conditions Stage Phenotype
jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 myelinating Schwann cell decreased amount, abnormal
jh4Tg; ue2Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 motor neuron myelination decreased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.