ZFIN Figure: Rocker et al., 2019, Fig. 6

ZFIN ID: ZDB-FIG-190716-20

ZFIN ID: ZDB-FIG-190716-20

Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation. American journal of physiology. Regulatory, integrative and comparative physiology 316(4):R395-R405 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Antibodies:	Ab1-tuba Ab2-sox10
Fish:	jh4Tg jh4Tg; ue2Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Anatomical Terms:	motor neuron axon myelin accumulating cell myelinating Schwann cell
Stage:	Days 7-13

PHENOTYPE:

Fish:	jh4Tg jh4Tg; ue2Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Observed In:	motor neuron myelination myelinating Schwann cell
Stage:	Days 7-13

Fig. 6

Acute hyperglycemia affects myelination and Schwann cell count along the motor nerve. A and B: lateral view of the spinal cord of control and metronidazole (MTZ)-treated ins:NTR;Tg(mbp:EGFP-CAAX) fish following the schedule of treatment described in Fig. 1A. NTR, nitroreductase; mbp, myelin basic protein; EGFP, enhanced green fluorescent protein. C: length of myelinated nerves was significantly shorter in MTZ-treated than control ins:NTR;Tg(mbp:EGFP-CAAX) fish. ****P < 0.0001. D and E: cross sections through ins:NTR zebrafish labeled with an antibody against Sox10 (red) and acetylated α-tubulin (green) in control and MTZ-treated fish. F: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads a modest decrease in Sox10⁺ cells (D′′ and E′′, arrows) along the motor axons (D′ and E′) in MTZ-treated compared with control fish. *P < 0.05. Scale bars = 50 μm.

Gene Expression Details

Gene	Antibody	Fish	Conditions	Stage	Anatomy	Assay
EGFP		jh4Tg; ue2Tg	control	Days 7-13	myelin accumulating cell	IFL
		jh4Tg; ue2Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	myelin accumulating cell	IFL

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab1-tuba	IHC	jh4Tg	control	Days 7-13	motor neuron axon
	IHC	jh4Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	motor neuron axon
Ab2-sox10	IHC	jh4Tg	control	Days 7-13	myelinating Schwann cell
	IHC	jh4Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	myelinating Schwann cell

Phenotype Details

Fish	Conditions	Stage	Phenotype
jh4Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	myelinating Schwann cell decreased amount, abnormal
jh4Tg; ue2Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	motor neuron myelination decreased occurrence, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.