FIGURE

Fig. 6

ID

ZDB-FIG-190716-20

Publication

Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation

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Fig. 6

Acute hyperglycemia affects myelination and Schwann cell count along the motor nerve. A and B: lateral view of the spinal cord of control and metronidazole (MTZ)-treated ins:NTR;Tg(mbp:EGFP-CAAX) fish following the schedule of treatment described in Fig. 1A. NTR, nitroreductase; mbp, myelin basic protein; EGFP, enhanced green fluorescent protein. C: length of myelinated nerves was significantly shorter in MTZ-treated than control ins:NTR;Tg(mbp:EGFP-CAAX) fish. ****P < 0.0001. D and E: cross sections through ins:NTR zebrafish labeled with an antibody against Sox10 (red) and acetylated α-tubulin (green) in control and MTZ-treated fish. F: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads a modest decrease in Sox10⁺ cells (D′′ and E′′, arrows) along the motor axons (D′ and E′) in MTZ-treated compared with control fish. *P < 0.05. Scale bars = 50 μm.

Expression Data

Gene:	EGFP
Antibodies:	Ab1-tuba Ab2-sox10
Fish:	jh4Tg jh4Tg; ue2Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Anatomical Terms:	motor neuron axon myelin accumulating cell myelinating Schwann cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh4Tg jh4Tg; ue2Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Observed In:	motor neuron myelination myelinating Schwann cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.