ZFIN ID: ZDB-FIG-190716-25
Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation. American journal of physiology. Regulatory, integrative and comparative physiology   316(4):R395-R405 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 1

Nitroreductase (NTR)-mediated β-cell ablation induces acute hyperglycemia. A: schematic outline of treatment schedule. MTZ, metronidazole. B and C: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads to selective ablation of pancreatic β-cells (C), which are unaffected by DMSO alone (B, red, arrow). D: following the schedule of treatment described in A, there was a significant difference in blood glucose levels in MTZ-treated compared with control fish. *P = 0.0239. E and F: lateral view of non-ins:NTRTg(nkx2.2a:megfp); Tg(NBT;DsRed) fish treated with DMSO or DMSO + 10 mM MTZ to determine possible off-target effects of MTZ. NTR, nitroreductase; NBT, neural-specific β-tubulin; megfp, monomeric enhanced green fluorescent protein; DsRed, Discosomared. No differences were observed. Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed vu16Tg; zf148Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 motor neuron axon IFL
EGFP vu16Tg; zf148Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 oligodendrocyte IFL
mCherry jh4Tg control Days 7-13 pancreatic B cell IFL
jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 Not Detected pancreatic B cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh4Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 7-13 pancreatic B cell absent, abnormal
Days 7-13 pancreatic B cell mCherry expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.