ZFIN Figure: Rocker et al., 2019, Fig. 1

ZFIN ID: ZDB-FIG-190716-25

ZFIN ID: ZDB-FIG-190716-25

Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation. American journal of physiology. Regulatory, integrative and comparative physiology 316(4):R395-R405 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed EGFP mCherry
Fish:	jh4Tg vu16Tg; zf148Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Anatomical Terms:	motor neuron axon oligodendrocyte pancreatic B cell
Stage:	Days 7-13

PHENOTYPE:

Fish:	jh4Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Observed In:	pancreatic B cell
Stage:	Days 7-13

Fig. 1

Nitroreductase (NTR)-mediated β-cell ablation induces acute hyperglycemia. A: schematic outline of treatment schedule. MTZ, metronidazole. B and C: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads to selective ablation of pancreatic β-cells (C), which are unaffected by DMSO alone (B, red, arrow). D: following the schedule of treatment described in A, there was a significant difference in blood glucose levels in MTZ-treated compared with control fish. *P = 0.0239. E and F: lateral view of non-ins:NTR, Tg(nkx2.2a:megfp); Tg(NBT;DsRed) fish treated with DMSO or DMSO + 10 mM MTZ to determine possible off-target effects of MTZ. NTR, nitroreductase; NBT, neural-specific β-tubulin; megfp, monomeric enhanced green fluorescent protein; DsRed, Discosomared. No differences were observed. Scale bar = 50 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
DsRed	vu16Tg; zf148Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13		motor neuron axon	IFL
EGFP	vu16Tg; zf148Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13		oligodendrocyte	IFL
mCherry	jh4Tg	control	Days 7-13		pancreatic B cell	IFL
	jh4Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	Not Detected	pancreatic B cell	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
jh4Tg	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole	Days 7-13	pancreatic B cell absent, abnormal
		Days 7-13	pancreatic B cell mCherry expression absent, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.