ZFIN ID: ZDB-FIG-190618-14
Borga et al., 2018 - Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Leukemia   33(2):333-347 Full text @ Leukemia
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 3

GFP fluorescence intensity of hMYC ALL correlates with B vs. T cell lineage. A qRT-PCR of ALL with differing GFP fluorescence (dim, n = 7; mixed, n = 6; bright, n = 14) of B cell-specific (ighm, ighz, pax5, cd79b; ab), T cell-specific (cd4, cd8a, il7r, lck; c-d), lymphoblast- specific (igic1s1, rag1, rag2; ef) genes and transgenes (GFP, d; hMYC, f). Results are normalized to housekeeping (HK) genes (β-actin and eef1a1l1) and shown as mean values ± S.D. Significant differences are indicated (Mann–Whitney test, p-values: ***<0.001, ****<0.0001). B Spearman correlation (p-value 0.0002, r = 0.82, r2 = 0.74) between lck vs. wMFI for: 7 B-ALL (circles), 6 mixed (triangles), and 4 T-ALL (squares). The solid line represents linear regression; dashed lines denote 95% confidence intervals. C Anti-GFP (#sc-9996) and anti-β-actin (#ab8227) WB of FACS-purified T- and pre-B ALL. D H&E of pre-B ALL infiltration in different sites and E hMYC control. F Anti-GFP (#GTX20290) IHC of hMYC pre-B ALL (pre-B2, left) and T-ALL (T-ALL1, right). 1000× images show staining with or without anti-GFP (Ab+; Ab-). th= thymus, ma= marrow, li= liver, mu= muscle, gi= gills, ey= eye, br= brain. 2× scale bar = 500 µm; 10× bar = 100 µm; 40× bar = 50 µm; 1000× bar = 20 µm

 

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cz2Tg; zdf14Tg standard conditions Adult head kidney hypotrophic, abnormal
Adult head kidney lymphoid progenitor cell infiltrative, abnormal
Adult liver lymphoid progenitor cell infiltrative, abnormal
Adult thymus lymphoid progenitor cell infiltrative, abnormal
Adult whole organism B-cell neoplasms increased amount, abnormal
Adult whole organism T-cell neoplasms increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Leukemia