ZFIN ID: ZDB-FIG-190618-12
Borga et al., 2018 - Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Leukemia   33(2):333-347 Full text @ Leukemia
Anatomical Term:
Stage: Adult
Observed In:
Stage: Adult

Fig. 1

Two ALL types inhMYC zebrafish with differing fluorescence intensities. AUnsupervised analysis of 10 hMYC (grey) and 3 hlk (magenta) ALL, using highest-variance probes. hMYC ALL cluster into hMYC-1 (blue) and hMYC-2 (orange) groups. Representative fluorescent images of fish with ALL from each group shown at upper left. B Venn diagram of 170 over-expressed genes in hMYC ALL compared to hlk T-ALL. Genes up-regulated by both hMYC-1 and −2 (n = 120) reside in the intersection, including six B cell-specific genes listed below the Venn diagram. Three other B cell-specific genes over-expressed by only hMYC-2 ALL are listed below the yellow circle. C Unsupervised analysis using B cell-specific genes. D Expression of ighm, blnk, pax5and itk in hlk, hMYC-1 and hMYC-2 ALL. Each gene is significantly differentially expressed in hlk T-ALL vs. hMYC-2 ALL (Mann–Whitney test, *p-value < 0.05). Expression values are log2 scale, normalized against the entire microarray dataset using the justRMA algorithm. Results shown as mean values ± standard deviation (S.D.). E Left: “bright” ALL, shown using low and high exposure settings (a, d). Cells are GFPhi by flow cytometry (g). Right: “dim” ALL, using low and high (c, f) exposures. Cells are GFPlo (i). Center: Fish with mixed-ALL (b, e), with discrete GFPlo and GFPhipopulations (h). F Images of control hMYC;GFP (upper) and lck:eGFP (lower) fish with only thymic (th) fluorescence


Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP cz2Tg standard conditions Adult thymus IFL
cz2Tg; zdf14Tg standard conditions Adult thymus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cz2Tg; zdf14Tg standard conditions Adult whole organism Lymphoid neoplasms increased amount, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Leukemia