FIGURE

Fig. 2

ID
ZDB-FIG-190524-2
Publication
Casar Tena et al., 2018 - Resting cells rely on the DNA helicase component MCM2 to build cilia
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Fig. 2

Loss of Mcm2 disrupts left-right asymmetry development. (A) Heart looping is randomized upon Mcm2 knockdown. Upper panel shows representative images of correct (D-loop) and aberrant heart looping (no loop, L-loop) at 48 hpf after in situ hybridization (ISH) for cmlc2. A, atrium; V, ventricle. Scale bar: 100 μm. Stacked bar graph summarizes heart looping experiments. D, D-loop; N, no loop; L, L-loop. P = 0.0003 (CTRL vs Mcm2 MO), P < 0.0001 (splCTRL vs Mcm2 splMO). n = 5–10 experiments. Number of embryos: NI = 309; CTRL = 166; Mcm2 MO = 128; splCTRL = 113; Mcm2 splMO = 194. (B) Example images of insulin (ins) ISH labelling of the endocrine pancreas (arrows). R, right (correct) or L, left (wrong position) of the embryonic midline. Scale bar: 100 μm.Graph displays quantification of aberrant pancreas position upon Mcm2 knockdown. P = 0.0052 (CTRL vs Mcm2 MO), P < 0.0001 (splCTRL vs Mcm2 splMO). n = 4–9 experiments. Number of embryos: NI = 299; CTRL = 146; Mcm2 MO = 109; splCTRL = 113; Mcm2 splMO = 192. (C) Loss of Mcm2 results in ambiguous southpaw (spaw) expression at 22 ss, which can be partially rescued by co-injection of mcm2 RNA. Upper panel shows examples of correct and aberrant spaw distribution. P < 0.0001 (splCTRL vs Mcm2 splMO), P = 0.0029 (splMO vs Mcm2 splMO + mcm2 RNA). n = 3 experiments. Number of embryos: NI = 156; splCTRL = 166; Mcm2 splMO = 152, mcm2 RNA = 142; Mcm2 splMO + mcm2 RNA = 181. (D) ML216-mediated inhibition of Bloom and Werner helicases from tailbud stage until 22ss does not affect asymmetry development. n = 3 experiments. Number of embryos: DMSO = 56; 1 μM ML216 = 61; 5 μM ML216 = 60; 10 μM ML216 = 57; 25 μM ML216 = 54; 50 μM ML216 = 53. All data analysed using two-tailed Fisher's exact test.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.