FIGURE

Fig. 2

ID

ZDB-FIG-180914-35

Publication

Cantaut-Belarif et al., 2018 - The Reissner Fiber in the Cerebrospinal Fluid Controls Morphogenesis of the Body Axis

Other Figures

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Fig. 2

Structural and Dynamic Properties of Cilia Appear Intact in the scospondin^icm13/icm13 Mutant

(A) Z projection of stacks of lateral optical sections (depth 4–5 μm) of spinal cord immunostained against acetylated tubulin show intact cilia projecting into the central canal of control and scospondin^icm13/icm13 larvae at 24 (top) and 30 hpf (bottom). In comparison, elipsa embryos exhibit fewer cilia at 24 hpf, which are not maintained at 30 hpf. Scale bars represent 15 μm.

(B) Time projection from a 30-s-long time series acquired at 17 Hz and indicating movement of cilia expressing GFP in Tg(β-actin:Arl13b-GFP; scospondin^icm13/icm13) animals (right) and control siblings (left). Note the similarity in position (posterior tilt, dashed lines) and beating amplitude of motile cilia in the central canal (arrowheads) in mutant embryos compared to control siblings. The schematic summarizes our observations. Rostral, left; dorsal, top. Scale bar represents 10 μm.

See also Figure S2 and Video S1.

Expression Data

Gene:	GFP
Antibody:	Ab1-tuba
Fish:	sspo^icm13/icm13 traf3ip1^tp49d/tp49d hsc5Tg sspo^icm13/icm13; hsc5Tg
Anatomical Terms:	central canal motile cilium ependymal cell motile cilium floor plate spinal cord region motile cilium
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sspo^icm13/icm13 traf3ip1^tp49d/tp49d sspo^icm13/icm13; hsc5Tg
Observed In:	ependymal cell epithelial cilium movement involved in extracellular fluid movement ependymal cell motile cilium spinal cord ependymal cell spinal cord motile cilium assembly
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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