ZFIN Figure: Asakawa et al., 2018, Fig. 4

ZFIN ID: ZDB-FIG-180913-55

ZFIN ID: ZDB-FIG-180913-55

Asakawa et al., 2018 - Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Cell Reports 24:1562-1572 Full text @ Cell Rep.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP RFP
Fish:	nkmnr2bhsga14Tg; nkuasrfp1aTg; vu12Tg mafba^{nkgsaizgffm35aGt/+}; nkhgj4aEt mafba^{nkgsaizgffm35aGt/+}; nkuasgfp1aTg mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkhgj4aEt mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkuasgfp1aTg
Anatomical Terms:	rhombomere 5 rhombomere 5 abducens motor nucleus rhombomere 5 neuroblast rhombomere 6 rhombomere 6 abducens motor nucleus rhombomere 6 neuroblast spinal cord motor neuron ventral root
Stage Range:	Prim-5 to Protruding-mouth

PHENOTYPE:

Fish:	mafba^{nkgsaizgffm35aGt/+}; nkhgj4aEt mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkhgj4aEt mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkuasgfp1aTg
Observed In:	rhombomere 5 rhombomere 5 abducens motor nucleus rhombomere 6 rhombomere 6 abducens motor nucleus
Stage Range:	Prim-5 to Protruding-mouth

Fig. 4

All mnr2b-ABNs Require mafba for Differentiation

(A) The mafba/valentino locus in Tg[gSAIzGFFM35A] line (val^M35A-Gal4 allele). Tg[gSAIzGFFM35A] (shown as M35A) is inserted in the coding exon of mafba (filled red box).

(B–E) Lateral (B and C) and dorsal (D and E) views of the hindbrain areas of Tg[UAS:GFP]; val^M35A-Gal4/+ and Tg[UAS:GFP]; val^{M35A-Gal4/M35A-Gal4} embryos at 24 hpf. The yellow brackets indicate the vestigial r5 and r6. Dashed lines and ovals show the midline and otic vesicle (ov), respectively.

(F and G) Dorsal views of the hindbrain and first spinal segment of Tg[mnr2b-GFP]; val^M35A-Gal4/+ (F) and Tg[mnr2b-GFP]; val^{M35A-Gal4/M35A-Gal4} (G) larvae at 80 hpf. The brackets and arrowheads indicate the dorsal and ventral mnr2b-ABNs, respectively. Asterisks in (G) show eGFP-expressing cells unrelated to mnr2b-ABNs. smn; spinal motor neuron; vr1; ventral root from the first spinal segment.

(H) Dorsal view of r5 and r6 in Tg[olig2:egfp]; Tg[mnr2b-hs:Gal4]; Tg[UAS:mRFP1] at 3 dpf.

(I and J) A single confocal section of the dorsal hindbrain in (H). (I) Arrows show mnr2b-ABNs (magenta) expressing eGFP derived from Tg[olig2:egfp] (J).

(K) Percentage of the mRFP1-expressing cells that expressed eGFP. In total, 78 and 87 cells in r5 and r6, respectively, were examined in five larvae.

Scale bars indicate 200 bp in (A), 100 μm in (B), 50 μm in (D)–(H), and 5 μm in (I) and (J).

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
EGFP	mafba^{nkgsaizgffm35aGt/+}; nkhgj4aEt	control	Protruding-mouth	Not Detected	rhombomere 5 abducens motor nucleus	IFL
			Protruding-mouth	Not Detected	rhombomere 6 abducens motor nucleus	IFL
			Protruding-mouth		spinal cord motor neuron	IFL
			Protruding-mouth		ventral root	IFL
	mafba^{nkgsaizgffm35aGt/+}; nkuasgfp1aTg	control	Prim-5		rhombomere 5	IFL
			Prim-5		rhombomere 6	IFL
	mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkhgj4aEt	control	Protruding-mouth	Not Detected	rhombomere 5 abducens motor nucleus	IFL
			Protruding-mouth	Not Detected	rhombomere 6 abducens motor nucleus	IFL
			Protruding-mouth		spinal cord motor neuron	IFL
			Protruding-mouth		ventral root	IFL
	mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkuasgfp1aTg	control	Prim-5		rhombomere 5	IFL
			Prim-5		rhombomere 6	IFL
	nkmnr2bhsga14Tg; nkuasrfp1aTg; vu12Tg	control	Protruding-mouth		rhombomere 5 neuroblast	IFL
			Protruding-mouth		rhombomere 6 neuroblast	IFL
RFP	nkmnr2bhsga14Tg; nkuasrfp1aTg; vu12Tg	control	Protruding-mouth		rhombomere 5 abducens motor nucleus	IFL
			Protruding-mouth		rhombomere 6 abducens motor nucleus	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
mafba^{nkgsaizgffm35aGt/+}; nkhgj4aEt	control	Protruding-mouth	rhombomere 5 abducens motor nucleus EGFP expression absent, abnormal
		Protruding-mouth	rhombomere 6 abducens motor nucleus EGFP expression absent, abnormal
mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkhgj4aEt	control	Protruding-mouth	rhombomere 5 abducens motor nucleus EGFP expression absent, abnormal
		Protruding-mouth	rhombomere 6 abducens motor nucleus EGFP expression absent, abnormal
mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkhgj4aEt	standard conditions	Protruding-mouth	rhombomere 5 abducens motor nucleus absent, abnormal
		Protruding-mouth	rhombomere 6 abducens motor nucleus absent, abnormal
mafba^{nkgsaizgffm35aGt/nkgsaizgffm35aGt}; nkuasgfp1aTg	control	Prim-5	rhombomere 5 vestigial, abnormal
		Prim-5	rhombomere 6 vestigial, abnormal

Construct: Gt(gSAIz-GAL4FFM)

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.