ZFIN Figure: Asakawa et al., 2018, Fig. 3

ZFIN ID: ZDB-FIG-180913-54

ZFIN ID: ZDB-FIG-180913-54

Asakawa et al., 2018 - Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Cell Reports 24:1562-1572 Full text @ Cell Rep.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP mRFP1 Tomato
Fish:	nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxgloxrTg nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxrloxgTg nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxgloxrTg nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxrloxgTg
Anatomical Terms:	abducens motor nucleus axon lateral rectus rhombomere 3 rhombomere 5 rhombomere 5 abducens motor nucleus rhombomere 6 rhombomere 6 abducens motor nucleus whole organism
Stage Range:	20-25 somites to Day 5

Fig. 3

mnr2b-ABNs in r5 and r6 Form Convergent Efferent Projections

(A) Structure of Tg[egr2b-Cre]. The Cre-polyA-Km^r cassette was inserted downstream of the 5′ UTR of egr2b.

(B and C) eGFP expression in a Tg[SAGFF73A]; Tg[egr2b-Cre]; Tg[UAS:loxRloxG] embryo at 20 hpf (C). Tg[SAGFF73A] transgene ubiquitously drives Gal4 from zfand5b promoter (B) (Asakawa and Kawakami, 2009).

(D and E) EGFP expression (D) driven by the combination of Tg[SAGFF73A], Tg[egr2b-Cre], and Tg[UAS:loxGloxR] transgenes (E).

(F, J, and N) Dorsal views of r5 and r6 in larvae carrying the transgenes indicated above at 5 dpf.

(G, K, and O) Schematic illustrations of Gal4- and Cre-mediated expression of eGFP and mRFP1 in r5 and r6.

(H, I, L, M, P, and Q) Axon terminals of mnr2b-ABNs innervating the left lateral rectus muscle. Tg[UAS:loxG] is generated by spontaneous excision of the floxed mRFP1 from Tg[UAS:loxRloxG] in the germline. The orange arrowheads indicate the terminal and boutons of en grappe terminals. The double arrowheads show the CPEZ. In (Q), mRFP1 signal in mnr2b-ABNs in r6 is below detection limit.

Scale bars indicate 200 bp in (A) and 50 μm in (C), (E), (F), and (H).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxgloxrTg	control	Day 5	abducens motor nucleus axon	IFL
			Day 5	rhombomere 6	IFL
			Day 5	rhombomere 6 abducens motor nucleus	IFL
	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxrloxgTg	control	Day 5	abducens motor nucleus axon	IFL
			Day 5	rhombomere 5	IFL
			Day 5	rhombomere 5 abducens motor nucleus	IFL
	nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxgloxrTg	control	20-25 somites	whole organism	IFL
	nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxrloxgTg	control	20-25 somites	rhombomere 3	IFL
			20-25 somites	rhombomere 5	IFL
mRFP1	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxgloxrTg	control	Day 5	rhombomere 5	IFL
	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxrloxgTg	control	Day 5	rhombomere 6	IFL
	nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxgloxrTg	control	20-25 somites	rhombomere 3	IFL
			20-25 somites	rhombomere 5	IFL
	nkegr2bcreTg; nkgsaizgffm773bGt; nkuasloxrloxgTg	control	20-25 somites	whole organism	IFL
Tomato	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxgloxrTg	control	Day 5	lateral rectus	IFL
	nkactc1btdTchrndTg; nkegr2bcreTg; nkmnr2bhsga14Tg; nkuasloxrloxgTg	control	Day 5	lateral rectus	IFL

Antibody Labeling Details No data available

Phenotype Details No data available

Construct: TgBAC(egr2b:Cre)

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.