FIGURE

Fig. S4

ID

ZDB-FIG-180720-41

Publication

Löhr et al., 2018 - Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit

Other Figures

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Fig. S4

Automated quantification of axon numbers in larval zebrafish brains. Related to Experimental Procedures.

(A-C) Anti-GFP IF on a Tg(pomca:EGFPras)^fr38Tg larvae at 14 dpf. (A) Confocal image series (z-series) of the preoptic area (POA). Yellow lines depict regions of interest for quantification of axonal innervation of the POA or anterior commissure (ac), respectively. (B) Generation of optical cross sections using the „Reslice“ tool, from Fiji Software (Image J, NIH) allows for visualization of single axons in the respective area. (C) Automated quantification of axons using the „Find Maxima“ function of Fiji Software. Quantified spots are indicated by yellow crosses.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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