ZFIN ID: ZDB-FIG-180511-52
Li et al., 2017 - Estrogen directly stimulates LHb expression at the pituitary level during puberty in female zebrafish. Molecular and Cellular Endocrinology   461:1-11 Full text @ Mol. Cell. Endocrinol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 45-89

Fig. 1

Gonad histology and real-time PCR analysis of related expression profiles of pituitary reproductive genes in peripubertal female zebrafish. Fish were grouped into PG (n = 7), PV (n = 6) or EV(n = 7) by their ovarian stages (A–C). A, only primary growth (PG) follicles could be found in the fish with body weight of 85.7 mg. B, previtellogenic (PV) stage follicles started to emerge in the fish with body weight of 111.5 mg. C, both early vitellogenic (EV) and PV stage follicles could be found in the fish of body weight of 134.8 mg. The expression profiles of lhb (D), fshb (E), cyp19a1b (F), esr1 (G), esr2a (H) and esr2b (I) in the pituitary were analyzed by real-time PCR. Relative expression was calculated using the comparative threshold (2−ΔΔCt) method. Data were present as mean ± SEM. Different letters above each bar denote significantly different. PG, primary growth; PV, previtellogenic follicle; EV, early vitellogenic follicle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp19a1b AB standard conditions Days 45-89 female organism hypophysis RTPCR
esr1 AB standard conditions Days 45-89 female organism hypophysis RTPCR
esr2a AB standard conditions Days 45-89 female organism hypophysis RTPCR
esr2b AB standard conditions Days 45-89 female organism hypophysis RTPCR
fshb AB standard conditions Days 45-89 female organism hypophysis RTPCR
lhb AB standard conditions Days 45-89 female organism hypophysis RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Molecular and Cellular Endocrinology, 461, Li, G., Tang, H., Chen, Y., Yin, Y., Ogawa, S., Liu, M., Guo, Y., Qi, X., Liu, Y., Parhar, I.S., Liu, X., Lin, H., Estrogen directly stimulates LHb expression at the pituitary level during puberty in female zebrafish, 1-11, Copyright (2017) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.