FIGURE

Fig. 5

ID

ZDB-FIG-180502-28

Publication

Chou et al., 2017 - A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish

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Fig. 5

Hair cell PCP and neuromast position govern dependence of mechanotransduction channel function on Tmc2b. a–f Confocal images of hair cells from L1, LII.1, and IO4 neuromasts of tmc2b ^+/− or tmc2b ^−/− animals. FM1-43FX (red) uptake reveals functional channels, and phalloidin (cyan) shows hair bundle polarity. Qualitative maps (yellow) of micrographs from tmc2b ^−/− mutants show that subpopulations of posterior- and ventral-facing hair bundles preferentially function (red) in L1 and LII.1, respectively; in contrast, hair cell uptake in IO4 is non-biased. g In PLL neuromasts with A–P orientations of tmc2b ^−/− mutants, the vast majority (n = 21) of FM1-43FX uptake is by hair bundles that are posterior facing (P-facing). Similarly, in the A–P-oriented neuromasts of the ALL of tmc2b ^−/− (O1, O2, OP1, and MI2), the overwhelming majority of FM1-43FX uptake is by P-facing hair cells (n ≥ 5, except for O1, n = 4). Red bars represent tmc2b ^−/−, and black bars signify tmc2b ^+/+ and tmc2b ^+/−. h In the PLL neuromasts with D–V orientations of tmc2b ^−/− mutants, ventral-facing (V-facing) hair cells dominate the population that uptakes the fluorophore (n = 16). In ALL neuromasts with D–V orientations of tmc2b ^−/−, the patterns of hair cells that take up FM1-43FX are complex. For IO4, there is no significant difference between the numbers of D- and V-facing hair cells that loaded with FM1-43FX. The FM1-43FX loading percentages are 82.6 ± 11.1 % (n = 5) for V-facing hair cells and 83.0 ± 11.1 % (n = 5) for D-facing hair cells in tmc2b ^−/−, respectively. One-way ANOVA with Holm-Sidak’s multiple comparisons test, P = 0.9986. D–V-oriented neuromasts MI1 and M2 have opposite PCP-related loading preferences in mutants. In MI1 and M2 of tmc2b ^−/− animals, the percentages of V-facing hair cells that load with FM1-43FX are 36.1 ± 3.4% (n = 6) and 2.9 ± 2.9% (n = 5), respectively. Whereas, the percentages of D-facing hair cells from MI1 and M2 that load with FM1-43FX are 7.9 ± 3.6% (n = 6) and 44.9 ± 8.3% (n = 5), respectively (see Supplementary Table 1 for statistics). Corresponding images of MI1 and M2 displayed in Supplementary Fig. 7. Scale bar = 5 μm. Arrowheads, immature hair cells that do not take up FM1-43FX. *dying hair cell

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmc2b^cwr8/cwr8 tmc2b^cwr8/+
Observed In:	infraorbital lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound mandibular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound middle lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound opercular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound otic lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound posterior lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound
Stage:	Day 6

Phenotype Detail

Acknowledgments

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