PUBLICATION

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish

Authors

Chou, S.W., Chen, Z., Zhu, S., Davis, R.W., Hu, J., Liu, L., Fernando, C.A., Kindig, K., Brown, W.C., Stepanyan, R., McDermott, B.M.

ID

ZDB-PUB-171223-3

Date

2017

Source

Nature communications 8: 2234 (Journal)

Registered Authors

Chou, Shih-wei, Davis, Robin Woods, Fernando, Carol, McDermott Jr., Brian M., Zhu, Shaoyuan (Sara)

Keywords

none

MeSH Terms

Lateral Line System/metabolism*
Lateral Line System/physiology
Water*
Mechanoreceptors/metabolism*
Mechanoreceptors/physiology
Animals
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Zebrafish Proteins/physiology
Zebrafish
Mechanotransduction, Cellular/genetics*
Mechanotransduction, Cellular/physiology
Membrane Proteins/genetics
Membrane Proteins/metabolism*
Membrane Proteins/physiology
Motion*

(all 16)

PubMed

29269857 Full text @ Nat. Commun.

Abstract

Detection of water motion by the lateral line relies on mechanotransduction complexes at stereocilia tips. This sensory system is comprised of neuromasts, patches of hair cells with stereociliary bundles arranged with morphological mirror symmetry that are mechanically responsive to two opposing directions. Here, we find that transmembrane channel-like 2b (Tmc2b) is differentially required for mechanotransduction in the zebrafish lateral line. Despite similarities in neuromast hair cell morphology, three classes of these cells can be distinguished by their Tmc2b reliance. We map mechanosensitivity along the lateral line using imaging and electrophysiology to determine that a hair cell's Tmc2b dependence is governed by neuromast topological position and hair bundle orientation. Overall, water flow is detected by molecular machinery that can vary between hair cells of different neuromasts. Moreover, hair cells within the same neuromast can break morphologic symmetry of the sensory organ at the stereocilia tips.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
cdh23^tj264/+	standard conditions	Day 6	posterior lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound process quality, normal	Fig. 3
cdh23^tj264/tj264	standard conditions	Day 6	posterior lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound decreased process quality, abnormal	Fig. 3
tmc2b^cwr8/+	standard conditions	Day 6	infraorbital lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 6
tmc2b^cwr8/+	standard conditions	Day 6	infraorbital lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 4
tmc2b^cwr8/+	standard conditions	Day 6	infraorbital lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 5
tmc2b^cwr8/+	standard conditions	Day 6	mandibular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 4
tmc2b^cwr8/+	standard conditions	Day 6	mandibular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 5
tmc2b^cwr8/+	standard conditions	Day 6	middle lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 4
tmc2b^cwr8/+	standard conditions	Day 6	middle lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 5
tmc2b^cwr8/+	standard conditions	Day 6	opercular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound occurrence, normal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
cwr1Tg	Tg(pvalb9:actb2-mCherry)	Transgenic Insertion
cwr8		Small Deletion	tmc2b
cwr9		Small Deletion	tmc2b
cwr10		Indel	tmc2a
tj264		Point Mutation	cdh23

1 - 5 of 5

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tmc2a	CRISPR1-tmc2a	CRISPR
tmc2b	CRISPR1-tmc2b	CRISPR
tmc2b	TALEN1-tmc2b	TALEN

Fish

Fish
cdh23^tj264/+
cdh23^tj264/tj264
cwr1Tg
tmc2b^cwr8/cwr8
tmc2b^cwr8/+
tmc2b^cwr9/cwr9; tmc2a^cwr10/cwr10
tmc2b^cwr9/+; tmc2a^cwr10/+

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab2-pvalb	polyclonal			Rabbit

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
mCherry	EFG	mCherry

Mapping

Chou et al., 2017 ZDB-PUB-171223-3 PMID:29269857

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish

Chou et al., 2017

ZDB-PUB-171223-3
PMID:29269857