FIGURE

Fig. S5

ID

ZDB-FIG-180126-28

Publication

Matsuoka et al., 2017 - CNS-resident progenitors direct the vascularization of neighboring tissues

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Fig. S5

VTA formation in vegfaa^bns1, vegfab^bns92, and vegfc^hu6410 mutants. (A) Predicted domain structure of WT Vegfab isoforms -171, -210, and Vegfab^bns92. Vegfab-171 and -210 consist of a signal peptide (SP), a knot motif, and heparin (Hep) and/or Hep/Nrp1 (Nrp) binding domains. vegfab^bns92 encodes a truncated peptide (p.Met88Trpfs*18). (B and C) 8 dpf Tg(hsp70l:vegfaa₁₆₅) vegfaa^−/− (B) and vegfc^−/− (C) larval trunk vasculature visualized by Tg(kdrl:EGFP) expression. Tg(hsp70l:vegfaa₁₆₅) vegfaa^−/− embryos were heat-shocked at 10 and 24 hpf to rescue their early vascular phenotype. VTAs are present in all of the vegfaa^−/− (B; n = 11) and vegfc^−/− (C; n = 10) larvae examined. (D and E) 8 dpf vegfab^+/+ (D) and vegfab^−/− (E) larval trunk vasculature visualized by FITC-dextran microangiography. vegfab^−/− larvae lack lumenized VTAs (yellow arrows, E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA