FIGURE

Fig. 6

ID
ZDB-FIG-170922-53
Publication
Xue et al., 2017 - The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Ccl25b Acts Downstream of Klf6a to Facilitate HSPC Settlement and Expansion

(A) Time-lapse imaging of kdrl:mCherry/runx1:en-GFP in control and ccl25b morphants. The white arrowheads mark HSPCs and the white circles indicate HSPC pockets. Scale bar, 50 μm.

(B) The statistical data of runx1+ cells in D/M/V of CVP in control and ccl25b morphants at 50 hpf (mean ± SD, t test; p < 0.05, ns [not significant] > 0.05, n = 8).

(C) Confocal imaging of kdrl:mCherry/runx1:en-GFP CHT at 3 dpf in control embryos and ccl25b morphants. Yellow arrowheads mark GFP+ cells. The white dashed lines mark the outlines of caudal artery and vein. Scale bar, 50 μm.

(D) The quantification of runx1:en-GFP+ cells shown in (C) (mean ± SD, t test; ∗∗p < 0.01, n = 8).

(E) Confocal imaging of FISH (cmyb)/immunofluorescence (PH3) at 60 hpf in CHT of control embryos and ccl25b morphants. White arrowheads mark double-positive cells. Scale bar, 50 μm.

(F) The statistical data showing that the percentage of double-positive/cmyb+ cells was reduced significantly in the morphants (mean ± SD, t test; ∗∗∗p < 0.001, n = 8).

(G) qPCR result of cell division-related gene expression in control and ccl25b morphants (mean ± SD, t test; p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns > 0.05, n = 3).

See also Figure S6.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 42(4), Xue, Y., Lv, J., Zhang, C., Wang, L., Ma, D., Liu, F., The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b, 349-362.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell