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Fig. 6

Ccl25b Acts Downstream of Klf6a to Facilitate HSPC Settlement and Expansion

(A) Time-lapse imaging of kdrl:mCherry/runx1:en-GFP in control and ccl25b morphants. The white arrowheads mark HSPCs and the white circles indicate HSPC pockets. Scale bar, 50 μm.

(B) The statistical data of runx1+ cells in D/M/V of CVP in control and ccl25b morphants at 50 hpf (mean ± SD, t test; p < 0.05, ns [not significant] > 0.05, n = 8).

(C) Confocal imaging of kdrl:mCherry/runx1:en-GFP CHT at 3 dpf in control embryos and ccl25b morphants. Yellow arrowheads mark GFP+ cells. The white dashed lines mark the outlines of caudal artery and vein. Scale bar, 50 μm.

(D) The quantification of runx1:en-GFP+ cells shown in (C) (mean ± SD, t test; ∗∗p < 0.01, n = 8).

(E) Confocal imaging of FISH (cmyb)/immunofluorescence (PH3) at 60 hpf in CHT of control embryos and ccl25b morphants. White arrowheads mark double-positive cells. Scale bar, 50 μm.

(F) The statistical data showing that the percentage of double-positive/cmyb+ cells was reduced significantly in the morphants (mean ± SD, t test; ∗∗∗p < 0.001, n = 8).

(G) qPCR result of cell division-related gene expression in control and ccl25b morphants (mean ± SD, t test; p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns > 0.05, n = 3).

See also Figure S6.

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Acknowledgments
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Reprinted from Developmental Cell, 42(4), Xue, Y., Lv, J., Zhang, C., Wang, L., Ma, D., Liu, F., The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b, 349-362.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell