FIGURE

Fig. 2

ID

ZDB-FIG-170921-89

Publication

Dash et al., 2016 - sept7b is required for the differentiation of pancreatic endocrine progenitors

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Fig. 2

Knockdown of sept7b reduces β-cell volume and inhibits β-cell proliferation.

(A,B) Three-dimensional rendered z-stack confocal images generated from control MO-injected (A) and sept7b TBMO-injected (B) zebrafish larva at 5 dpf stained as whole mounts with antibodies against insulin. (C) Insulin-positive cell volume is significantly reduced in sept7b TBMO-injected zebrafish larvae at 5 dpf. (D,E) Three-dimensional rendered z-stack confocal images generated from control MO-injected (D) and sept7b TBMO-injected (E) zebrafish larva at 5 dpf treated with EdU (green) to visualize proliferating cells and stained with antibodies against insulin (red) to visualize β-cells. DAPI shows the nuclei (blue). Arrowheads indicate proliferating β-cells (positive for both EdU and insulin). (F) β-cell proliferation is significantly reduced in sept7b knockdown larvae. Error bars represent mean ± SEM. Scale bar (A,B) (10 μm); (D,E) (10 μm). ***p ≤ 0.0005.

Expression Data

Gene:	ins
Antibody:	Ab1-ins
Fish:	WT WT + MO1-septin15
Knockdown Reagent:	MO1-septin15
Anatomical Term:	pancreatic B cell cytoplasm
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-septin15
Knockdown Reagent:	MO1-septin15
Observed In:	endocrine pancreas pancreatic B cell pancreatic B cell cell population proliferation
Stage:	Day 5

Phenotype Detail

Acknowledgments

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