FIGURE

Fig. S2

ID

ZDB-FIG-170109-9

Publication

Takeuchi et al., 2015 - Type IV Collagen Controls the Axogenesis of Cerebellar Granule Cells by Regulating Basement Membrane Integrity in Zebrafish

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Fig. S2

Sparse cell labeling of GC axons in col4a6 mutants.

Sparse cell labeling was performed by injecting UAS:Kaede reporter DNA and Tol1 transposase RNA into the GC-specific Gal4 line hspGFFDMC90A. Immunostaning with anti-Kaede (A, B, D, E, G, H) and anti-Neurod antibodies (A, D, G). 5-dpf wild-type (A, B) and col4a6 (D, E, G, H) larvae. Dorsal views. Arrowhead shows abnormal GC axons (E, H). (C, F, I) Schematic drawing of normal GC axons (C) and typical abnormal axons of GCs in LCa (I), EG and CCe (F). (J) Percentage of abnormal and normal GC axons in wild-type and the col4a6 mutant larvae. Statistic analysis is shown in S1 Table. The GC axons were significantly affected in the col4a6 mutant larvae, compared to wild-type larvae (Fisher’s exact test p<0.01 for LCa and EG, and p<0.05 for CCe). Most of the GC axons from the LCa and EG displayed misorientation, whereas only a portion of the GC axons in the CCe were affected. Scale bars: 50 μm in A (applied to B, D, E, G, H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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