FIGURE

Fig. 3

ID

ZDB-FIG-161201-3

Publication

Shin et al., 2016 - Vegfa signals through ERK to promote angiogenesis, but not artery differentiation

Other Figures

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Fig. 3

Chemical inhibition of ERK blocks ISV sprouting. (A-C) Transmitted light images of embryos treated with the indicated compound and concentration from 10 hpf to 24 hpf. Arrows in B indicate necrosis. (D) Western blot analysis of pERK, total ERK and actin in embryos treated from 24 to 25 hpf as indicated. (E-H) Transmitted light images (E,F) and confocal images (G,H) of Tg(cdh5:gal4ff)^mu101;(uas:nTom)^um152;(uas:egfp)^nkuasgfpa1 embryos at 32 hpf. Embryos were treated with 0.1% DMSO (E,G) or 15 µM SL327 (F,H) from 20 to 32 hpf. DA and PCV are denoted by brackets in G,H and shorter ISVs are indicated by arrows in H. (I-K) Quantification of ISV length (I), cell number per ISV (J) and BrdU-positive cells per ISV (K) following treatment with the indicated compound. *P<0.05, ***P<0.001; ns, not statistically significant; numbers of ISVs and embryos counted across three experiments are indicated on the x-axis. Error bars represent s.d.

Expression Data

Genes:	GFP Tomato
Fish:	mu101Tg; nkuasgfp1aTg; um152Tg
Condition:	chemical treatment by environment: SL-327
Anatomical Terms:	dorsal aorta endothelial cell intersegmental vessel endothelial cell posterior cardinal vein endothelial cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT mu101Tg; nkuasgfp1aTg; um152Tg
Condition:	chemical treatment by environment: SL-327
Observed In:	intermediate cell mass of mesoderm intersegmental vessel intersegmental vessel endothelial cell tail bud
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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