FIGURE

Fig. 3

ID
ZDB-FIG-161003-2
Publication
Matsuda et al., 2016 - Epb41l5 competes with Delta as a substrate for Mib1 to coordinate specification and differentiation of neurons
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Fig. 3

Delays in disassembly of AJs and neuronal differentiation in epb41l5 morphants. (A-H′) Timeline of AJ disassembly (A-D′) and differentiation of neurons (E-H2) during DAPT treatment in wild-type embryos. Embryos were treated with 50µM DAPT for 1.5, 3 or 6h and immunostained at 32hpf. Loss of Notch signaling by DAPT treatment results in loss of AJs and premature differentiation of NPCs into neurons. (I-P′) Delays in AJ disassembly and differentiation of neurons in epb41l5 embryos. Embryos were treated with DAPT for 5h. In control embryos (WT), 5h DAPT treatment eliminates ZO-1 at the apical/ventricular surface, accompanied by a corresponding increase in HuC. In epb41l5 morphants, there is a much smaller reduction in ZO-1 immunostaining and no obvious increase in HuC immunostaining. (Q,R) Quantitative analyses of ZO-1 and HuC expression. Total fluorescence intensities of ZO-1 and HuC in the hindbrain were measured in individual confocal images using ImageJ. Error bars represent s.d. n.s., not significant. M, million integrated pixel intensity.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
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