FIGURE
Fig. S1
- ID
- ZDB-FIG-160819-31
- Publication
- Sako et al., 2016 - Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation
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Fig. S1
Embryo light exposure and subcellular localization of Opto-acvr 1b and 2b. (A-D) Tg(gsc:GFP) embryos at bud stage (10 hpf) either kept in the dark (A, C) or exposed to blue LED light (B, D; 5.12 µW/mm2, see material and methods) from 2-cell to bud stage (0.510 hpf); upper (A, B) and lower (C, D) panels show bright-field and fluorescent images, respectively; scale bar, 200 µm. (EH) Anti- HA (Opto-acvr 1b- or 2bHA), phalloidin (filamentous actin), and DAPI (nuclei) staining of cells at the animal pole of optoacvr 1b or 2b mRNA (20 pg each)- injected MZoep embryos at high stage (3 hpf); scale bar, 20 µm. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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