FIGURE

Fig. 7

ID
ZDB-FIG-160819-26
Publication
Halloum et al., 2016 - Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent
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Fig. 7

Virulence of ΔMAB_4780 is attenuated in a neutrophil-depleted host. (A) Maximum intensity projection of confocal images showing representative neutrophil activity (at 2 dpi) in pu.1 Tg(mpx:egpf) morphants i.v.-infected with Mabs R, ΔMAB_4780, or complemented strain expressing tdTomato. (B) Csf3r Tg(mpx:egpf) morphants showed highly reduced neutrophil numbers at 3 and 4 dpf, confirming the proper activity of the morpholino. The numbers of mpx-positive neutrophils counted in the trunk and tail at 3 dpf are depicted in the graph. (C) Survival curve of neutrophil-depleted larvae i.v.-infected with the Mabs R, ΔMAB_4780, and complemented strains (~150 cfu, n = 20 per group). These larvae were hypersusceptible to Mabs R and to the complemented strain (P < 0.001, log-rank test) but not to ΔMAB_4780 (statistically not different from PBS-injected controls). Shown are representative data from two independent experiments. (D) Representative fluorescence and DIC overlays of infected csf3r morphants at 2 and 3 dpi.

Expression Data
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA
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