Morpholino

MO3-spi1b

ID

ZDB-MRPHLNO-071203-2

Name

MO3-spi1b

Previous Names

MO3-spi1

Target

spi1b (1)

Sequence

5' - GGTCTTTCTCCTTACCATGCTCTCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

splice-blocker targeted to exon 4-5 boundary

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO3-spi1b

Expressed Gene	Anatomy	Figures
csf1ra	(not) myeloid cell pigment cell	Fig. 4 from Su et al., 2007
lcp1	(not) intermediate cell mass of mesoderm	Fig. 4 from Su et al., 2007
lyz	granulocyte	Fig. 4 from Su et al., 2007
mpx	granulocyte	Fig. 4 from Su et al., 2007

Phenotype

Phenotype resulting from MO3-spi1b

Phenotype	Fish	Figures
macrophage differentiation disrupted, abnormal	WT + MO3-spi1b	Fig. 4 from Su et al., 2007
myeloid cell decreased amount, abnormal	WT + MO3-spi1b	Fig. 4 from Su et al., 2007

Phenotype of all Fish created by or utilizing MO3-spi1b

Phenotype	Fish	Conditions	Figures
whole organism decreased life span, ameliorated	AB + MO3-spi1b	bacterial treatment by injection: Burkholderia cenocepacia	Fig. 1 from Mesureur et al., 2017
defense response to bacterium disrupted, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 3 from Clay et al., 2007
defense response to bacterium decreased occurrence, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 1 , Fig. 6 from Clay et al., 2008
whole organism lacks all parts of type macrophage, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 5 from Clay et al., 2007
macrophage absent, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 6 from Clay et al., 2008
defense response to bacterium disrupted, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 4 from Clay et al., 2007
defense response to bacterium disrupted, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 5 from Clay et al., 2007
whole organism lacks all parts of type macrophage, abnormal	WIK + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	text only from Clay et al., 2007
whole organism viability, abnormal	WT + MO2-spi1b + MO3-spi1b	bacterial treatment: Streptococcus pneumoniae TIGR4	Fig. 5 from Rounioja et al., 2012
whole organism decreased life span, abnormal	WT + MO2-spi1b + MO3-spi1b	bacterial treatment: Streptococcus pneumoniae TIGR4	Fig. 5 from Rounioja et al., 2012
myeloid cell decreased amount, abnormal	WT + MO3-spi1b	standard conditions	Fig. 4 from Su et al., 2007
macrophage differentiation disrupted, abnormal	WT + MO3-spi1b	standard conditions	Fig. 4 from Su et al., 2007
granuloma formation increased occurrence, abnormal	i114Tg + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacteroides abscessus	Fig. 7 from Halloum et al., 2016
whole organism decreased life span, exacerbated	ump2Tg + MO2-spi1b + MO3-spi1b	bacterial treatment by injection: Mycobacteroides abscessus	Fig. 5 from Bernut et al., 2015
macrophage absent, abnormal	ump2Tg + MO2-spi1b + MO3-spi1b	control	Fig. 5 from Bernut et al., 2015
macrophage mCherry expression absent, abnormal	ump2Tg + MO2-spi1b + MO3-spi1b	control	Fig. 5 from Bernut et al., 2015
macrophage mCherry expression absent, abnormal	ump2Tg + MO3-spi1b	bacterial treatment by injection: Burkholderia cenocepacia	Fig. 1 from Mesureur et al., 2017
macrophage absent, abnormal	ump2Tg + MO3-spi1b	bacterial treatment by injection: Burkholderia cenocepacia	Fig. 1 from Mesureur et al., 2017
macrophage decreased amount, abnormal	ump2Tg + MO3-spi1b	bacterial treatment by injection: Burkholderia cenocepacia	Fig. 1 from Mesureur et al., 2017
defense response to bacterium disrupted, abnormal	y1Tg + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacterium marinum	Fig. 6 from Clay et al., 2007
whole organism decreased life span, abnormal	slc24a5^b1/b1 + MO2-spi1b + MO3-spi1b	bacterial treatment: Mycobacteroides abscessus	Fig. 6 from Halloum et al., 2016

Citations