FIGURE

Fig. S8

ID

ZDB-FIG-160311-27

Publication

Guo et al., 2016 - Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation

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Fig. S8

Failure to rescue defective convergent extension and body curvature in lbx1b-overexpressing embryos by Rac1 mRNA injection.

(A) Dorsal view of dlx3b/hgg1/ntl expression in the tail bud of embryos injected with buffer (ctrl), lbx1b mRNA (lbx1b), or lbx1b and human RAC1 mRNA (lbx1b+Rac1). (B) Quantitative analysis of convergent extension (CE) movement with embryos in (A) (ctrl, n = 15; lbx1b, n = 15; lbx1b+Rac1, n = 13). The extent of defective CE was evaluated by measuring the distance between the inner edges of bilateral dlx3b expression at 3 regions as indicated by the arrows in panel A. RAC1 mRNA injection failed to rescue defective CE. *p < 0.01, NS: not significant. (C) Dorsal view of 48 hpf Tg(hsp:Gal-VP; EGFP:UAS:lbx1b) embryos upon heat shock at 4 hpf with buffer (lbx1b) or RAC1 mRNA (lbx1b+Rac1) injection. (D) Quantitative analysis of body curvature with the embryos in (C) (lbx1b, n = 11; lbx1b+Rac1, n = 11). No significant change of the severity of body curvature was observed in RAC1 mRNA-injected embryos. NS: not significant. Severity of body curvature was quantified by the angle as described in Fig 3. F2 lines of driver and responder transgenic fish were used. The dosages for embryo injections were 50 pg/embryo for lbx1b mRNA and 40 pg/embryo for RAC1 mRNA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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