FIGURE

Fig. 2

ID

ZDB-FIG-160304-63

Publication

McPherson et al., 2016 - Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

th2⁺ Cells Are Continuously Generated throughout Life

(A) 5 dpf larvae were treated with BrdU for 24 hr and analyzed at 9 dpf. Numerous th2:GFP⁺ cells (green) are labeled with BrdU (magenta) in the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(B) 12 dpf larvae were treated with BrdU for 24 hr and analyzed at 25 dpf. th2:GFP⁺ cells (green) labeled with BrdU (magenta) can be found in medial regions of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(C) 6 mpf fish were injected interperitoneally with BrdU and analyzed 2 weeks later. th2:GFP⁺ cells (green) labeled with BrdU (magenta) can be found in a midsagittal view of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(D) Percent of BrdU⁺ cells in the th2:GFP⁺ population throughout the entire posterior recess. Error bars represent SEM; n = 5 brains.

Images in (A) and (B) are ventral maximum intensity confocal z projections of the hypothalamic posterior recess. Images in (C) are maximum intensity confocal z projections from midsagittal views of the hypothalamic posterior recess. Individual and merged channels of boxed region are shown in the lower panels. The scale bars represent 10 µM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.