FIGURE

Fig. S4

ID

ZDB-FIG-151106-18

Publication

Ju Shin et al., 2015 - Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Selective depletion of nuclear GFP by Ab-SPOP E3 ligases engineered with other nanobodies against GFP. 293TetOn cells expressing GFP were transfected with vectors expressing four different Ab-SPOP_ΔNLS ligases (GBP1-SPOP, GBP2-SPOP, GBP6-SPOP, and GBP7- SPOP) from a bi-directional TRE promoter. Expression of TagRFP and depletion of GFP were measured 8 hours after adding doxycycline (1 µg/ml) to media. ′-′ indicates untransfected cells. Yellow arrowhead show cells where nuclear, not cytoplasmic GFP is depleted.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.