FIGURE

Fig. S7

ID

ZDB-FIG-151106-21

Publication

Ju Shin et al., 2015 - Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins

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Fig. S7

Reversibility of Ab-SPOP. 293TetOn cells expressing H2B-GFP from a CMV promoter and Ab- SPOP/TagRFP from a bi-directional TRE promoter recover H2B-GFP signal after doxycycline withdrawal. (a) Cells grown for 60 hours in medium without doxycycline. (b) Cells grown for 60 hours in medium containing doxycycline (1 µg/ml). Medium was changed every 24 hours. (c) Cells treated with doxycycline (1 µg/ml) for 12 hours, and then changed to fresh medium containing no doxycycline. Medium was changed every 24 hours. After 48 hours in medium without doxycycline, the H2B-GFP signal was partially recovered. (d) Protein extracts from the cells treated with doxycycline (–: w/o doxycycline for 60 hours, +: w/ doxycycline for 60 hours, +: w/ doxycycline for 12 hours and then with fresh medium for 48 hours) were analyzed by immunoblotting with anti-GFP, anti-FLAG, and anti-Actin antibodies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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