FIGURE

Fig. S6

ID

ZDB-FIG-151106-20

Publication

Ju Shin et al., 2015 - Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins

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Fig. S6

Time-course of H2B-GFP depletion by Ab-SPOP. 293TetOn cells expressing H2B-GFP were transfected with vectors expressing Ab-SPOP ligase (vhhGFP4-SPOP) or Ab_mutation-SPOP from bi- directional TRE promoters. Expression of TagRFP and depletion of nuclear H2B-GFP were measured after adding doxycycline (1 µg/ml) to media. Yellow arrowheads show nuclei with depletion of H2B-GFP. TagRFP was first detected about 8 hours after doxycycline-mediated TRE promoter activation. Depletion of nuclear H2B-GFP started 3 hours after TRE promoter activation and was observed in cells transfected with Ab-SPOP but not with Ab_mutationSPOP.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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