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Fig. S4
Selective depletion of nuclear GFP by Ab-SPOP E3 ligases engineered with other nanobodies against GFP. 293TetOn cells expressing GFP were transfected with vectors expressing four different Ab-SPOPΔNLS ligases (GBP1-SPOP, GBP2-SPOP, GBP6-SPOP, and GBP7- SPOP) from a bi-directional TRE promoter. Expression of TagRFP and depletion of GFP were measured 8 hours after adding doxycycline (1 µg/ml) to media. ′-′ indicates untransfected cells. Yellow arrowhead show cells where nuclear, not cytoplasmic GFP is depleted.
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