FIGURE

Fig. 2

ID

ZDB-FIG-151102-51

Publication

Jahr et al., 2015 - Hyperspectral light sheet microscopy

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Fig. 2

Image formation and virtual filtering.

(a) Schematic drawing of a two days post fertilization zebrafish embryo imaged laterally. (b) Typical x, λ-data sets acquired along three different lines in a Tg(h2afva:h2afva-GFP,kdrl:Hsa.HRAS-mCherry) zebrafish embryo. Spatial information is displayed horizontally, spectral information vertically. Dark horizontal stripes (arrowheads) were caused by the QuadNotch filter blocking scattered illumination light. Scale bar, 100nm (spectral) × 100µm (spatial). (c) Spectra were extracted from the x, λ-data sets by integration along the x axis. From the spectra, regions that could be assigned unambiguously to a colour channel were chosen and integrated (′virtual filtering′). (d) Reconstruction of a dual channel image with cyan (left): 475-555nm, red (right): 601-718nm. Scale bar, 100µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.