ZFIN Image: Jahr et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Image formation and virtual filtering.

(a) Schematic drawing of a two days post fertilization zebrafish embryo imaged laterally. (b) Typical x, λ-data sets acquired along three different lines in a Tg(h2afva:h2afva-GFP,kdrl:Hsa.HRAS-mCherry) zebrafish embryo. Spatial information is displayed horizontally, spectral information vertically. Dark horizontal stripes (arrowheads) were caused by the QuadNotch filter blocking scattered illumination light. Scale bar, 100nm (spectral) × 100µm (spatial). (c) Spectra were extracted from the x, λ-data sets by integration along the x axis. From the spectra, regions that could be assigned unambiguously to a colour channel were chosen and integrated (′virtual filtering′). (d) Reconstruction of a dual channel image with cyan (left): 475-555nm, red (right): 601-718nm. Scale bar, 100µm.

Acknowledgments

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