FIGURE

Fig. 6

ID

ZDB-FIG-151102-54

Publication

Jahr et al., 2015 - Hyperspectral light sheet microscopy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Linear unmixing of five fluorophores.

(a) For imaging five colours, wild-type fish stained with Hoechst or Bodipy as well as fish expressing only EGFP, eYFP or dsRed were imaged to obtain reference spectra. Black arrowheads highlight regions where the signal is suppressed by the QuadNotch Filter. (b) A zebrafish embryo expressing Tg(kdrl:EGFP,s1013t:Gal4,UAS:ChR2-eYFP,ptf1a:dsRed) was stained with Hoechst and Bodipy and imaged dorsally. Pseudo-colour overlay after unmixing the hyperspectral data set with reference spectra. For better visualization, Bodipy intensity is reduced to 75%. (c) Individual channels, from left to right: Hoechst (blue), EGFP (green), eYFP (yellow), dsRed (magenta) and Bodipy (red). Maximum intensity projections of 65 planes, z-spacing 2µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.