FIGURE

Fig. 4

ID

ZDB-FIG-150929-34

Publication

He et al., 2013 - Trans-2-phenylcyclopropylamine regulates zebrafish lateral line neuromast development mediated by depression of LSD1 activity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

High concentration of 2-PCPA treatment leads to apoptosis. (A-E) 2-PCPA treatment at high concentration (150 µM) induced an enhancement of cellular apoptosis within neuromasts. A-B: AO staining in the neuromast of a control (A) and a 2-PCPA treated larva (B) at 5 dpf. White arrows indicated AOpositive cells. (C) Cleaved Caspase-3 staining in the neuromast of a 150 µM 2-PCPA treated larva at 5 dpf. (D) The detection of hair cell death in neuromast. Neuromast images from brn3c:mGFP larvae treated with 150 µM 2-PCPA. White arrows in (D) showed dying hair cells being extruded from the neuromast. Scale bar =10 µm. (E) 150 µM 2-PCPA treated larva (lower panel) have high levels of acridine orange (AO) staining (white arrows) in neuromasts, compared with control (upper panel) and 75 µM 2-PCPA treated larva (middle panel) at 5 dpf.

Expression Data

Gene:	GFP
Fish:	s356tTg
Condition:	chemical treatment: tranylcypromine hydrochloride
Anatomical Term:	neuromast
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: tranylcypromine hydrochloride
Observed In:	neuromast apoptotic process
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.