FIGURE

Fig. 6, S1

ID

ZDB-FIG-150826-13

Publication

Auer et al., 2015 - Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3

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Fig. 6, S1

Silencing of all Retinal Ganglion Cells by BoTx expression leads to Ntf3 upregulation.

(A) Expression of botulinum toxin light chain B (Brunger et al., 2008; Nevin et al., 2008) in all RGCs leads to expansion of melanosomes similar to blind mutant fish as shown in Figure 1—figure supplement 1. Scale bar = 200 µm. (B) Relative expression levels of ntf3 in 4-dpf old wildtype, kif5aa mutant, and Tg(Isl2b:Gal4, UAS:BoTxLCB-GFP) transgenic embryos. ntf3 is upregulated to a similar extent in Tg(Isl2b:Gal4, UAS:BoTxLCB-GFP) transgenic embryos as in kif5aa mutants. (C) Western blotting confirms the overexpression of Ntf3 protein in embryos without presynaptic activity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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