ZFIN ID: ZDB-FIG-150826-12
Auer et al., 2015 - Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 5 Kif5aa mutant RGC arbors show the same density of presynaptic sites but are depleted of mitochondria.

(A) In vivo imaging shows the distribution of presynaptic sites marked by Synaptophysin-GFP (SypGFP) in single kif5aa mutant and wild-type RGC arbors expressing membrane localized RFP (RFPCaax). Upper panel: wild-type cell arbor, lower panel: kif5aa mutant cell arbor. Scale bars = 20 µm. D = dorsal, V = ventral, R = rostral, C = caudal. (B) Upper panel: Zoom in to an axonal segment indicated in the right panel of (A). Stable presynaptic clusters of SypGFP larger than 0.4 µm were defined as synapses (white arrows in the upper panel) and synapse density in axonal segments of wild-type and mutant cell arbors does not show a significant difference at 5 and 7 dpf (lower panel). (C) Distribution of mitochondria (labeled by mitoGFP) in single mutant and wild-type RGC arbors expressing membrane localized RFP (RFPCaax) in vivo. Upper panel: wild-type cell arbor, lower panel: kif5aa mutant cell arbor. Mutants RGC cell arbors show substantially less mitochondria. Scale bars = 20 µm. (D) Transmission electron micrograph of a transvers section of the neuropil containing RGC axonal arbors. Upper panel: wild-type neuropil, lower panel: kif5aa mutant neuropil. In yellow circles: mitochondria. Left panel: Zoom in into a single axonal segment containing a mitochondrion. The kif5aa mutant neuropil contains less mitochondria. Scale bar = 500 nm. (E) Quantification of mitochondria area per neuropil area comparing wild-type and mutant tecta at 6 dpf. Mutant cells contain significantly less mitochondria than wild-type cells (p < 0.01).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II presynapse IFL
Day 5 retinal ganglion cell presynapse IFL
kif5aaic3023/ic3023; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II presynapse IFL
Day 5 retinal ganglion cell presynapse IFL
kif5aaic3024/ic3024; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II presynapse IFL
Day 5 retinal ganglion cell presynapse IFL
mRFP ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
kif5aaic3023/ic3023; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
kif5aaic3023/ic3023; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
kif5aaic3024/ic3024; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
kif5aaic3024/ic3024; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II axolemma IFL
Day 5 retinal ganglion cell axon collateral IFL
Day 5 retinal ganglion cell axon terminus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kif5aaic3023/ic3023 standard conditions Day 5 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
Day 6 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 6 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 6 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
Days 7-13 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Days 7-13 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Days 7-13 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
kif5aaic3023/ic3023; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 retinal ganglion cell axon terminus has normal numbers of parts of type retinal ganglion cell presynapse, normal
Days 7-13 retinal ganglion cell axon terminus has normal numbers of parts of type retinal ganglion cell presynapse, normal
kif5aaic3023/ic3023; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
kif5aaic3024/ic3024 standard conditions Day 5 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
Day 6 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 6 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 6 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
Days 7-13 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Days 7-13 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Days 7-13 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
kif5aaic3024/ic3024; ic3021Tg; ic3025Tg; tpl2Tg standard conditions Day 5 retinal ganglion cell axon terminus has normal numbers of parts of type retinal ganglion cell presynapse, normal
Days 7-13 retinal ganglion cell axon terminus has normal numbers of parts of type retinal ganglion cell presynapse, normal
kif5aaic3024/ic3024; ic3025Tg; tpl2Tg standard conditions Day 5 cranial nerve II anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell anterograde axonal transport of mitochondrion decreased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus has fewer parts of type retinal ganglion cell mitochondrion, abnormal
Acknowledgments:
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