ZFIN ID: ZDB-FIG-150826-7
Auer et al., 2015 - Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Fig. 3 RGC axons in kif5aa mutants show a delayed ingrowth into the optic tectum and grow larger arbors at later stages.

(A) Single membrane-GFP expressing RGC axons from the Tg(BGUG) transgene (left panel) and DiI injections into the contralateral retina of 3 dpf old wild-type and kif5aa mutant embryos (right panel) illustrate the delay of tectal innervation in mutants. Scale bars = 20 µm. The schematic in the lower left panel illustrates the perspective chosen for image acquisition (indicated by an arrow). D = dorsal, V = ventral, R = rostral, C = caudal. (B) Upper panel, left: Axonal arbor of a single wild-type RGC at 3, 5, and 7 dpf. Lower panel, left: Tracings of an axonal arbor at time point zero. In red: Overlay of filopodia formed and retracted within 10 min (1 frame/2 min). Scale bars = 20 µm. Upper panel, right: Axonal arbor of a single kif5aa mutant RGC axonal arbor at 3, 5, and 7 dpf. Lower panel, right: Tracing of an axonal arbor at time point zero. In red: Overlay of filopodia formed and retracted within 10 min (1 frame/2 min). Scale bars = 20 µm. (C) Kif5aa mutant RGC axons grow significantly more branches, cover a larger area of the optic tectum with their arbors (in µm2) and grow longer arbors (in µm) than wild-type cells at 7 dpf (n = 10, p < 0.01). Scale bars = 20 µm. For quantification, only branches stable within 10 min of image acquisition were selected. (D) Quantification of filopodia numbers formed and retracted within 10 min per cell at 3, 5, and 7 dpf. While wild-type RGC arbors form most filopodia at 3 dpf and reduce this rate constantly until 7 dpf, kif5aa mutant RGC axons grow almost three times more filopodia at 5 dpf (n = 4, p < 0.01). At 7 dpf, the rate is still more as double as high as for their wild-type counterparts (n = 4, p < 0.01).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP kif5aaic3023/ic3023; s318tTg standard conditions Protruding-mouth retinal ganglion cell axon IFL
Day 5 retinal ganglion cell axon IFL
Days 7-13 retinal ganglion cell axon IFL
kif5aaic3024/ic3024; s318tTg standard conditions Protruding-mouth retinal ganglion cell axon IFL
Day 5 retinal ganglion cell axon IFL
Days 7-13 retinal ganglion cell axon IFL
s318tTg standard conditions Protruding-mouth retinal ganglion cell axon IFL
Day 5 retinal ganglion cell axon IFL
Days 7-13 retinal ganglion cell axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kif5aaic3023/ic3023; s318tTg standard conditions Protruding-mouth cranial nerve II axonogenesis involved in innervation delayed, abnormal
Protruding-mouth cranial nerve II collateral sprouting decreased occurrence, abnormal
Protruding-mouth retinal ganglion cell axon terminus decreased branchiness, abnormal
Day 5 cranial nerve II collateral sprouting increased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus increased branchiness, abnormal
Days 7-13 cranial nerve II collateral sprouting increased occurrence, abnormal
Days 7-13 retinal ganglion cell axon terminus increased branchiness, abnormal
kif5aaic3024/ic3024; s318tTg standard conditions Protruding-mouth cranial nerve II axonogenesis involved in innervation delayed, abnormal
Protruding-mouth cranial nerve II collateral sprouting decreased occurrence, abnormal
Protruding-mouth retinal ganglion cell axon terminus decreased branchiness, abnormal
Day 5 cranial nerve II collateral sprouting increased occurrence, abnormal
Day 5 retinal ganglion cell axon terminus increased branchiness, abnormal
Days 7-13 cranial nerve II collateral sprouting increased occurrence, abnormal
Days 7-13 retinal ganglion cell axon terminus increased branchiness, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife