ZFIN ID: ZDB-FIG-150810-11
Evason et al., 2015 - Identification of Chemical Inhibitors of β-Catenin-Driven Liver Tumorigenesis in Zebrafish. PLoS Genetics   11:e1005305 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig. 3 Activated β-catenin causes larval liver enlargement and increased hepatocyte proliferation.

(A) Brightfield images of control sibling and transgenic 6-day-old fixed larvae. Livers are outlined. Scale bars, 100 µm. (B) Graph showing average liver size ± SEM of 6-day-old larvae from three different transgenic lines (s985, s986, s987) compared to control siblings (C). N values are shown above the x-axis. Asterisks indicate p-values for 2-way ANOVA comparing transgenic zebrafish to control siblings in the same experiment: **, p<0.01; ***, p<0.001. (C) Immunofluorescence images of 6-day-old control and transgenic larvae, highlighting hepatocyte cell membranes (Tg(fabp10a:rasGFP)) and proliferating cells (EdU). Scale bars, 20 µm. Inset photos are 4X magnifications. (D-E) Graphs showing average cell size ± SEM (D) and percent of hepatocytes that were EdU positive (E). N values, which are the same for both graphs, appear above the x-axis in D; samples were compared using the Student’s t-test. *, p<0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s704Tg; s942Tg standard conditions Day 6 hepatocyte plasma membrane IHC
s942Tg standard conditions Day 6 hepatocyte plasma membrane IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s702Tg standard conditions Day 6 liver increased size, abnormal
s703Tg standard conditions Day 6 liver increased size, abnormal
s704Tg standard conditions Day 6 liver increased size, abnormal
s704Tg; s942Tg standard conditions Day 6 hepatocyte size, normal
Day 6 liver cell population proliferation increased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.