ZFIN Figure: Bührdel et al., 2015, Fig. 2

ZFIN ID: ZDB-FIG-150609-16

ZFIN ID: ZDB-FIG-150609-16

Bührdel et al., 2015 - In vivo characterization of human myofibrillar myopathy genes in zebrafish. Biochemical and Biophysical Research Communications 461(2):217-23 Full text @ Biochem. Biophys. Res. Commun.

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	WT + MO1-cryaba WT + MO1-cryabb WT + MO1-dnajb6a WT + MO1-dnajb6b WT + MO1-fhl1b WT + MO1-ldb3a WT + MO1-myot WT + MO1-plcg1 WT + MO1-pleca WT + MO1-plecb WT + MO2-bag3 WT + MO2-cmlc1 WT + MO2-desma WT + MO2-flnca WT + MO3-desmb WT + MO3-fhl1a WT + MO3-vcp WT + MO4-flncb
Knockdown Reagents:	MO1-cryaba MO1-cryabb MO1-dnajb6a MO1-dnajb6b MO1-fhl1b MO1-ldb3a MO1-myot MO1-plcg1 MO1-pleca MO1-plecb MO2-bag3 MO2-cmlc1 MO2-desma MO2-flnca MO3-desmb MO3-fhl1a MO3-vcp MO4-flncb
Observed In:	locomotion thigmotaxis whole organism
Stage Range:	Prim-5 to Protruding-mouth

Fig. 2

Loss of MFM gene function results in a progressive motility deficiency (A–C) Spontaneous movement assay with false-colored superimposed overviews of 24 hpf control (A) and MFM morphants (B–C); Red pictures = 0 s; green pictures = 10 s. (D) Summary of voluntary movement at 24 hpf of MFM morphants. (E) Quantification of flight reaction to a touch stimulus of 72 hpf individuals. Darker gray bars indicate the effect of Morpholinos directed against non-MFM genes as controls. Asterisks indicate significance compared to the MO-control group.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-cryaba	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-cryabb	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-dnajb6a	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-dnajb6b	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-fhl1b	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-ldb3a	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-myot	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-plcg1	standard conditions	Prim-5	locomotion process quality, normal
		Prim-5	whole organism mobility, normal
		Protruding-mouth	thigmotaxis process quality, normal
WT + MO1-pleca	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO1-plecb	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO2-bag3	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO2-cmlc1	standard conditions	Prim-5	locomotion process quality, normal
		Prim-5	whole organism mobility, normal
		Protruding-mouth	thigmotaxis process quality, normal
WT + MO2-desma	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO2-flnca	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO3-desmb	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO3-fhl1a	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO3-vcp	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal
WT + MO4-flncb	standard conditions	Prim-5	locomotion disrupted, abnormal
		Prim-5	whole organism decreased mobility, abnormal
		Protruding-mouth	thigmotaxis disrupted, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.