ZFIN ID: ZDB-FIG-150609-16
Bührdel et al., 2015 - In vivo characterization of human myofibrillar myopathy genes in zebrafish. Biochemical and Biophysical Research Communications   461(2):217-23 Full text @ Biochem. Biophys. Res. Commun.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Loss of MFM gene function results in a progressive motility deficiency (A–C) Spontaneous movement assay with false-colored superimposed overviews of 24 hpf control (A) and MFM morphants (B–C); Red pictures = 0 s; green pictures = 10 s. (D) Summary of voluntary movement at 24 hpf of MFM morphants. (E) Quantification of flight reaction to a touch stimulus of 72 hpf individuals. Darker gray bars indicate the effect of Morpholinos directed against non-MFM genes as controls. Asterisks indicate significance compared to the MO-control group.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cryaba standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-cryabb standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-dnajb6a standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-dnajb6b standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-fhl1b standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-ldb3a standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-myot standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-plcg1 standard conditions Prim-5 locomotion process quality, normal
Prim-5 whole organism mobility, normal
Protruding-mouth thigmotaxis process quality, normal
WT + MO1-pleca standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO1-plecb standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO2-bag3 standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO2-cmlc1 standard conditions Prim-5 locomotion process quality, normal
Prim-5 whole organism mobility, normal
Protruding-mouth thigmotaxis process quality, normal
WT + MO2-desma standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO2-flnca standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO3-desmb standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO3-fhl1a standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO3-vcp standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
WT + MO4-flncb standard conditions Prim-5 locomotion disrupted, abnormal
Prim-5 whole organism decreased mobility, abnormal
Protruding-mouth thigmotaxis disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.